Abstract
The research paper presented herein explores the chemical analysis of curcumin and piperine utilizing high-performance liquid chromatography (HPLC) techniques. The investigation encompasses the identification of appropriate solvents for solubility testing, method development, and subsequent validation of the HPLC method for the precise quantification of curcumin and piperine. A diverse range of chemicals, solvents, and instruments, notably the LC-2030C 3D Plus IND HPLC system, were employed to execute the analytical procedures. The primary objectives of the research were to optimize the mobile phase composition, establish suitable chromatographic conditions, and validate the method to ensure accuracy, precision, linearity, and specificity. Method optimization entailed meticulous experimentation to determine the most effective combination of mobile phase components, flow rates, and detection wavelengths to achieve optimal separation and detection of curcumin and piperine peaks. Subseque